Chromosome-Scale Genome Assembly of <i>Fusarium oxysporum</i> Strain Fo47, a Fungal Endophyte and Biocontrol Agent
Bo Wang, Houlin Yu, Yanyan Jia, Quanbin Dong, Christian Steinberg, Claude Alabouvette, Véronique Edel‐Hermann, Harold Kistler, Kai Ye, Li‐Jun Ma, Li Guo
Abstract
Here, we report a chromosome-level genome assembly of Fusarium oxysporum Fo47 (12 pseudomolecules; contig N 50 : 4.52 Mb), generated using a combination of PacBio long-read, Illumina paired end, and high-throughput chromosome conformation capture sequencing data. Although F. oxysporum causes vascular wilt to over 100 plant species, the strain Fo47 is classified as an endophyte and is widely used as a biocontrol agent for plant disease control. The Fo47 genome carries a single accessory chromosome of 4.23 Mb, compared with the reference genome of F. oxysporum f. sp. lycopersici Fol4287. The high-quality assembly and annotation of the Fo47 genome will be a valuable resource for studying the mechanisms underlying the endophytic interactions between F. oxysporum and plants as well as for deciphering the genome evolution of the F. oxysporum species complex.