The Development of Immunological Assays to Evaluate the Level and Function of Antibodies Induced by Klebsiella pneumoniae O-Antigen Vaccines
Helen R. Wagstaffe, Marina Johnson, Guled A. Osman, Patricia Martín, Paula Carranza, David Goldblatt
Abstract
There is no licensed vaccine for the prevention of Klebsiella pneumoniae infections, and increasing levels of antibiotic resistance make this pathogen a high priority for vaccine and therapeutic development. Standardized assays for testing vaccine immunogenicity are paramount for the development of vaccines, and so in this study, we optimized and standardized both antibody-level and function assays for evaluating in-development K. pneumoniae bioconjugate vaccine response in rabbits.
Topics & Concepts
Klebsiella pneumoniaeImmunogenicityAntibodyMicrobiologyVirologyVaccinationAntigenKlebsiellaBiologyPathogenSerotypeImmunologyMedicineEscherichia coliGeneBiochemistryAntibiotic Resistance in BacteriaBacterial Infections and VaccinesEscherichia coli research studies