Evaluation of the Properties of the DNA Methyltransferase from Aeropyrum pernix K1
Mao Hayashi, Keisuke Sugahara, Akira Yamamura, Yasuhiro Iida
Abstract
In general, thermophilic bacteria with optimum growth temperatures over or equal to 60°C have been predicted to include only N 4 -methylcytosine or N 6 -methyladenine as methylated bases in their DNA, because 5-methylcytosine is susceptible to deamination by heat. However, from this study, A. pernix K1, with an optimum growth temperature at 95°C, was demonstrated to produce a DNA (cytosine-5)-methyltransferase.
Topics & Concepts
CytosineMethyltransferaseThermophileMethylationGuanineDNABiologyDNA methyltransferaseEscherichia coliRestriction enzymePrimer (cosmetics)genomic DNAGeneticsChemistryNucleotideGeneBacteriaOrganic chemistryEpigenetics and DNA MethylationRNA modifications and cancerProtist diversity and phylogeny