Nanoscale Polyion Complex Vesicles for Delivery of Cargo Proteins and Cas9 Ribonucleoprotein Complexes to Plant Cells
Masaki Odahara, K. Watanabe, Riku Kawasaki, Kousuke Tsuchiya, Ayaka Tateishi, Yoko Motoda, T. Kigawa, Yutaka Kodama, Keiji Numata
Abstract
DNA-free genome editing using Cas9 ribonucleoprotein is advantageous because it introduces fewer potential undesirable genetic modifications than comparable methods. Here, we show a direct protein delivery system for plants using a cell-penetrating peptide-displayed polyion complex vesicle, CPP-PICsome, as a biological nanocarrier. With this system, we demonstrate nuclear genome editing by delivery of a Cas9 ribonucleoprotein complex in Arabidopsis thaliana callus.
Topics & Concepts
RibonucleoproteinGenome editingCas9CRISPRNanocarriersCell biologyGuide RNAComputational biologyGenomeBiologyHeterogeneous nuclear ribonucleoproteinChemistryGeneRNAGeneticsDrug deliveryOrganic chemistryRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic Engineering