Qingyi decoction attenuates intestinal epithelial cell injury <i>via</i> the calcineurin/nuclear factor of activated T-cells pathway
Guanyu Wang, Dong Shang, Guixin Zhang, Huiyi Song, Nan Jiang, Huan‐Huan Liu, Hailong Chen
Abstract
BACKGROUND: Recent studies have demonstrated that dysfunction of the intestinal barrier is a significant contributing factor to the development of severe acute pancreatitis (SAP). A stable intestinal mucosa barrier functions as a major anatomic and functional barrier, owing to the balance between intestinal epithelial cell (IEC) proliferation and apoptosis. There is some evidence that calcium overload may trigger IEC apoptosis and that calcineurin (CaN)/nuclear factor of activated T-cells (NFAT) signaling might play an important role in calcium-mediated apoptosis. AIM: To investigate the potential mechanisms underlying the therapeutic effect of Qingyi decoction (QYD) in SAP. METHODS: studies, Caco-2 cells were treated with lipopolysaccharide (LPS) and QYD serum, and then cell viability and intracellular calcium levels were detected. RESULTS: levels and inhibited cell death. CONCLUSION: the CaN/NFATc3 pathway.