Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging
Assel Baibek, Muhammed Üçüncü, Bryn Short, Gordon Ramage, Annamaria Lilienkampf, Mark Bradley
Abstract
The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.
Topics & Concepts
FluorescenceAmphotericin BDyeingChemistryMicrobiologyAntifungalBiologyOrganic chemistryOpticsPhysicsAntifungal resistance and susceptibilityPhotodynamic Therapy Research Studiesbioluminescence and chemiluminescence research