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Decoding the Tumor Microenvironment: Exosome-Mediated Macrophage Polarization and Therapeutic Frontiers

Yilin Li, Jiaqi You, Zifang Zou, Guanghao Sun, Yuqing Shi, Yanbin Sun, Shun Xu, Xin Zhang

2025International Journal of Biological Sciences33 citationsDOIOpen Access PDF

Abstract

CD8+ T cells, fibroblasts) was also evaluated, which indicated their roles in immune evasion and metastasis. Further, engineering strategies, such as receptor-targeted exosomes and short palindromic repeats interference (CRISPRi)-based transcriptional silencing, were also discussed as emerging tools to enhance exosome specificity and therapeutic efficacy. This study proposes a roadmap for translating engineered exosomes into clinical immunotherapy regimens by integrating recent advances in spatial omics and artificial intelligence, and also addresses challenges in exosome isolation, stability, and biosafety.

Topics & Concepts

ExosomeMacrophage polarizationTumor microenvironmentMacrophageCancer researchDecoding methodsChemistryCell biologyComputational biologyBiologyMicrovesiclesTumor cellsComputer sciencemicroRNABiochemistryIn vitroGeneTelecommunicationsExtracellular vesicles in disease