An ESIPT-induced NIR fluorescent probe to visualize mitochondrial sulfur dioxide during oxidative stress <i>in vivo</i>
Haixian Ren, Fangjun Huo, Xia Wu, Xiaogang Liu, Caixia Yin
Abstract
Based on the change in electron distribution of the benzopyrylium unit before and after sulfite addition, a 2-(2'-hydroxyphenyl)benzothiazole (HBT)-based fluorophore generated the excited state intramolecular proton transfer (ESIPT) process with a near-infrared enhanced emission at 836 nm and a large Stokes shift (286 nm). The probe was applied to image SO2 derivatives in cells and mice. Our data will provide new ideas for the development of ESIPT-based fluorescence probe with longer wavelength emission.
Topics & Concepts
In vivoFluorescenceOxidative stressSulfur dioxideChemistryBiophysicsOxidative phosphorylationSulfurPhotochemistryBiochemistryBiologyOrganic chemistryOpticsGeneticsPhysicsSulfur Compounds in BiologySulfur-Based Synthesis TechniquesMolecular Sensors and Ion Detection