PET imaging of focused-ultrasound enhanced delivery of AAVs into the murine brain
Javier Ajenjo, Jai Woong Seo, Josquin Foiret, Bo Wu, Marina N. Raie, James Wang, Brett Z. Fite, Nisi Zhang, R.S. Malek, Corinne Beinat, Noeen Malik, David Anders, Katherine W. Ferrara
Abstract
Combining microbubbles with US-guided treatment facilitated a multi-hour BBB disruption and stable AAV transduction in targeted areas of the murine brain. This unique platform has the potential to provide insight and aid in the translation of AAV-based therapies for the treatment of neuropathologies.
Topics & Concepts
Transduction (biophysics)Gene deliveryReporter geneGenetic enhancementEx vivoIn vivoAdeno-associated virusMicrobubblesMedicineTransgeneBlood–brain barrierPreclinical imagingNeurosciencePathologyBiologyCentral nervous systemVector (molecular biology)Gene expressionGeneUltrasoundBiophysicsGeneticsRecombinant DNARadiologyVirus-based gene therapy researchViral gastroenteritis research and epidemiologyUltrasound and Hyperthermia Applications