Highly modular hepatitis B virus-like nanocarriers for therapeutic protein encapsulation and targeted delivery to triple negative breast cancer cells
Daniel Yur, Millicent O. Sullivan, Wilfred Chen
Abstract
SpyCatcher-SpyTag bioconjugation and a multi-expression system, respectively. This new platform facilitates modification with epidermal growth factor receptor (EGFR)-targeting proteins and encapsulation with both model green fluorescent protein (GFP) and prodrug-converting yeast cytosine deaminase (yCD) enzyme. The resultant targeted VLPs demonstrated enhanced uptake and toxicity in EGFR-overexpressing triple negative breast cancer (TNBC) cells in contrast to non-malignant breast epithelial cells.
Topics & Concepts
NanocarriersTriple-negative breast cancerEncapsulation (networking)Cancer researchBreast cancerMaterials scienceNanotechnologyDrug deliveryMedicineCancerComputer scienceInternal medicineComputer networkGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies ResearchBiochemical and Structural Characterization