Cancer Cell Macrophage Membrane Camouflaged Persistent Luminescent Nanoparticles for Imaging-Guided Photothermal Therapy of Colorectal Cancer
Zhi‐Hao Wang, Jingmin Liu, Ning Zhao, Chunyang Li, Shi‐Wen Lv, Yaozhong Hu, Huan Lv, Di Wang, Shuo Wang
Abstract
Colorectal cancer is one of the leading causes of cancer incidence and mortality worldwide. Early diagnosis and treatment of colorectal cancer is beneficial to improve the therapeutic efficacy and prolong the survival time of patients. In recent years, persistent luminescence nanoparticles (PLNPs) have attracted more and more attention in the field of biomedical imaging due to the long persistent luminescence, zero autofluorescence, and high signal-to-noise ratio. To real-time trace and diagnose colorectal cancer and produce a precise treatment effect, herein, Zn 1.25 Ga 1.5 Ge 0.25 O 4:Cr 3+,Yb 3+,Er 3+ (ZGGO) PLNPs were prepared and served as the trackable center and coated with mesoporous silica (ZGGO@SiO 2 ). Afterward, photothermal fluorescent dye IR825 and chemotherapeutic drug irinotecan (Ir) were successively loaded into the mesoporous ZGGO@SiO 2 and encapsulated with a cancer cell–macrophage hybrid membrane. With relatively uniform particle size, the hybrid membrane camouflaged nano delivery system (IR825/Ir ZGGO@SiO 2 @CMM) exhibited superior escape ability and homologous adhesion ability in in vitro cellular uptake experiment and in vivo persistent luminescence imaging experiment, favoring the long-term retaining of nanocarriers in vivo and targeted accumulating in tumor sites. In vivo antitumor experiment showed that the nanoplatform could produce precise combined colorectal cancer chemotherapy and imaging-guided photothermal therapy under the persistent luminescent guide of PLNPs. After 3 weeks of treatment, the relative tumor volume of CT26-tumor-bearing mice treated with IR825/Ir ZGGO@SiO 2 @CMM and laser irradiation was only one-fifth of that of uncoated IR825/Ir ZGGO@SiO 2 . With superior immune escape ability and tumor homologous adhesion ability, the cancer cell–macrophage hybrid membrane camouflaged nanomaterials may provide a universal nanoplatform that allows integrating various therapy modalities for precise cancer treatment of a biomimetic nano delivery system.