Description of Halosolutus amylolyticus gen. nov., sp. nov., Halosolutus halophilus sp. nov. and Halosolutus gelatinilyticus sp. nov., and genome-based taxonomy of genera Natribaculum and Halovarius
Yaping Sun, Beibei Wang, Xi-Wen Zheng, Zhang-Ping Wu, Jing Hou, Heng‐Lin Cui
Abstract
Three extremely halophilic archaeal strains (LT55 T , SQT-29-1 T and WLHS5 T ) were isolated from Gobi saline soil and a salt lake, China. These strains were most related to the genera Natribaculum and Halovarius (92.6–95.1 % similarities), and showed low similarities with other genera within the family Natrialbaceae based on 16S rRNA genes. Phylogenomic analysis confirmed that the three strains formed a distinct clade separated from the related genera Halostagnicola and Natronococcus , which indicated that they may represent a novel genus of the family Natrialbaceae . The average nucleotide identity (ANI), in silico DNA–DNA hybridization ( is DDH) and average amino acid identity (AAI) values among the three strains were no more than 87, 34 and 85 %, respectively, much lower than the threshold values for species demarcation. The major phospholipids of the three strains were phosphatidic acid (PA), phosphatidylglycerol (PG) and phosphatidylglycerol phosphate methyl ester (PGP-Me). The glycolipid profiles of the three strains were diverse; sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S 2 -DGD) were found in strains LT55 T and WLHS5 T , while mannosyl glucosyl diether (DGD-1) and S-DGD-1 in strain SQT-29-1 T . The combination of phenotypic, chemotaxonomic, phylogenetic and genomic analyses suggested that strains WLHS5 T (=CGMCC 1.13781 T = JCM 33558 T ), SQT-29-1 T (=CGMCC 1.16065 T = JCM 33554 T ) and LT55 T (=CGMCC 1.15188 T = JCM 30838 T ) represent three novel species of a new genus within the family Natrialbaceae , for which the names, Halosolutus amylolyticus gen. nov., sp. nov., Halosolutus gelatinilyticus sp. nov. and Halosolutus halophilus sp. nov., are proposed. Genome-based classification of genera Natribaculum and Halovarius revealed that Halovarius luteus should be transferred to the genus Natribaculum as Natribaculum luteum comb. nov. and Natribaculum longum as a heterotypic synonym of Natribaculum breve Liu et al . 2015.