Alkaline phosphatase activity as a biochemical biomarker in aqua-toxicological studies

Abstract

Alkaline phosphatase (ALP) is a glycoprotein with a metallophosphatase structure that catalyzes the hydrolysis of monophosphate esters of biomolecule esters at alkaline pH.ALP activity is a useful bioindicator to assess the physiological health of cellular membranes, cell growth, apoptosis and cell migration, cellular metabolic status, hepatocyte function, and detoxification activity in hepatocytes.ALP activity is detected in a colorimetric method using the para-nitrophenyl phosphate substrate (p-NPP) at a wavelength of 405 nm in biological samples.Cell hemolysis, especially erythrocytes; increased levels of sex hormones and corticosteroids, biological infections, and poor nutrition can adversely affect ALP activity.

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