Self-Sorting Collagen Heterotrimers
Valdrin Islami, Philipp Bittner, Tomas Fiala, Nina B. Hentzen, Renato Zenobi, Helma Wennemers
Abstract
Nature uses elaborate methods to control protein assembly, including that of heterotrimeric collagen. Here, we established design principles for the composition and register-selective assembly of synthetic collagen heterotrimers. The assembly code enabled the self-sorting of eight different strands into three─out of 512 possible─triple helices via complementary (4 S )-aminoproline and aspartate residues. Native ESI-MS corroborated the specific assembly into coexisting heterotrimers.
Topics & Concepts
ChemistryHeterotrimeric G proteinTriple helixBiophysicsSortingBiochemistryStereochemistryG proteinSignal transductionAlgorithmComputer scienceBiologyCollagen: Extraction and CharacterizationProteins in Food SystemsEnzyme Production and Characterization