3D Printed Porous Nanocellulose-Based Scaffolds As Carriers for Immobilization of Glycosyltransferases
F. Lackner, Hui Liu, Andreja Dobaj Štiglic, Matej Bračič, Rupert Kargl, Bernd Nidetzky, Tamilselvan Mohan, Karin Stana Kleinschek
Abstract
-SuSy), corresponding to an efficacy of 37 and 18%, respectively, compared to the soluble enzymes. The glycosyltransferases were coimmobilized and shown to be active in a cascade reaction to give the natural C-glycoside nothofagin from phloretin (1.0 mM; ∼95% conversion). All enzyme bound scaffolds showed reusability of a maximum of 5 consecutive reactions. These results suggest that the 3D printed and cross-linked NFC/CMC-based scaffolds could present a class of solid carriers for enzyme (co)-immobilization, with promising applications in glycosyltransferase-catalyzed synthesis and other fields of biocatalysis.
Topics & Concepts
NanocelluloseChemistryGlycosyltransferaseImmobilized enzymeCelluloseNucleotide sugarSelf-healing hydrogelsScaffoldCarboxymethyl celluloseEnzymeChemical engineeringBiochemistryOrganic chemistryMedicineBiomedical engineeringSodiumEngineeringEnzyme Catalysis and ImmobilizationEnzyme Production and CharacterizationGlycosylation and Glycoproteins Research