An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates
Debbie Pankratz, Nicolas Gomez, Agnes Nielsen, Ayten Mustafayeva, Melisa Gür, Fabián Arce-Rodriguez, Pablo I. Nikel, Susanne Häußler, Alejandro Arce‐Rodríguez
Topics & Concepts
RecombineeringSynthetic biologyBiologyCRISPRCas9RecombinaseComputational biologyPlasmidGenome editingContext (archaeology)Pseudomonas aeruginosaGenome engineeringGeneticsMicrobial geneticsDNABacteriaGeneRecombinationPaleontologyCRISPR and Genetic EngineeringBacterial biofilms and quorum sensingVibrio bacteria research studies