Fluorescent sensor for in-vivo bio-imaging, precise tracking of Fe3+ ions in Zebrafish embryos and visual measuring of Cu2+ ions in pico-molar level
A. Senthil Murugan, Mathiyalagan Kiruthika, Enose Rajan Abel Noelson, P. Yogapandi, G. Gnana kumar, J. Annaraj
Abstract
Feasibly synthesized novel rhodamine based chemosensor (RS) has been described for the detection of paramagnetic and biologically important Cu2+ and Fe3+ ions. Among all the transition metal ions, this chemosensor was able to precisely spot out the Cu2+/Fe3+ ion couple by its spirolactam cleavage, consequently enhanceing their absorbances at 521 and 529 nm for Cu2+ and Fe3+ ions respectively even at low concentrations [263 pM (Cu2+) and 2 nM (Fe3+)], which is prominently lower than the WHO and the United States Environmental Protection Agency (USEPA) strategies. This metal pair (Cu2+/Fe3+) has more tendencies to cleave the lactam ring even in the presence of higher concentrations of other relevant metal ions. Such remarkable behaviour of RS has been motivated us to apply zebrafish bio-imaging and molecular logic circuits.