ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis
Eleonora Bailoni, Bert Poolman
Abstract
glycerol kinase GlpK to synthesize glycerol 3-phosphate from externally supplied glycerol. We fuel phospholipid headgroup formation by sustainable l-arginine breakdown. In addition, we design and characterize a dynamic dialysis setup optimized for synthetic cells, which is used to control the external medium composition and to achieve sustainable glycerol 3-phosphate synthesis.
Topics & Concepts
LipidomeGlycerolPhospholipidSynthetic biologyBiochemistryChemistryMetabolic engineeringGlycerol kinaseCompartmentalization (fire protection)BiophysicsEnzymeLipid metabolismBiologyMembraneBioinformaticsOrigins and Evolution of LifePhotoreceptor and optogenetics researchProtein Structure and Dynamics