QuantiFERON-TB Gold Plus with Chemiluminescence Immunoassay: Do We Need a Higher Cutoff?
Francesco Bisognin, Giulia Lombardi, Maria Carla Re, Paola Dal Monte
Abstract
= 0.8728), but CLIA produced higher values than ELISA. Receiver operating characteristic (ROC) analysis showed that the optimal cutoff value in CLIA was 0.45 IU/ml for TB1 and 0.46 IU/ml for TB2. In conclusion, automated QFT-Plus with CLIA is comparable to QFT-Plus performed by ELISA. Within the linearity range of the test, CLIA detects higher quantitative values than ELISA, resulting in a higher number of determinate results and the conversion of samples that were close to the cutoff into positive borderline results. A higher cutoff for QFT-CLIA needs to be defined based on clinical diagnostic criteria.
Topics & Concepts
ChemiluminescenceImmunoassayGold standard (test)Interferon γLatent tuberculosisTuberculosisMedicineMycobacterium tuberculosisInterferon gammaQuantiFERONImmunologyChemistryChromatographyInternal medicinePathologyAntibodyCytokineTuberculosis Research and EpidemiologyMycobacterium research and diagnosisInfectious Diseases and Tuberculosis