A traceless linker for aliphatic amines that rapidly and quantitatively fragments after reduction
Maomao He, Jie Li, Hesong Han, Clarissa A. Borges, Gabriel Neiman, Joachim Justad Røise, Piotr Hadaczek, Rima Mendonsa, Victoria R. Holm, Ross C. Wilson, Krystof S. Bankiewicz, Yumiao Zhang, Corinne M. Sadlowski, Kevin E. Healy, Lee W. Riley, Niren Murthy
Abstract
to Cas9 with DEC was able to generate a self-delivering Cas9 RNP that could edit cells without transfection reagents. Finally, conjugation of donor DNA to Cas9 with DEC increased the homology directed DNA repair (HDR) rate of the Cas9 RNP by 50% in HEK 293T cell line. We anticipate that DEC will have numerous applications in biotechnology, given the ubiquitous presence of aliphatic amines on small molecule and protein therapeutics.
Topics & Concepts
LinkerChemistryCombinatorial chemistryCleavage (geology)Reduction (mathematics)IntracellularBiochemistryComputer scienceMaterials scienceComposite materialFracture (geology)Operating systemGeometryMathematicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMolecular Junctions and Nanostructures