Olive and green tea leaves extract in Pleurotus ostreatus var. florida culture media: Effect on mycelial linear growth rate, diameter and growth induction index
W Werghemmi, Sami Abou Fayssal, H Mazouz, Hassan Hajjaj, Lhoussain Hajji
Abstract
Abstract The exponential disposal of agro-industrial wastes onto the environment has endangered all forms of life. The implementation of these wastes in mushroom production is an eco-friendly and promising solution. The effect of olive and tea leaves extracts represented in culture media treatments: potato dextrose agar (PDA) 80% + tea extract (TE) 20% (T2), PDA 80% + olive extract (OE) 20% (T3), PDA 80% + OE 10% + TE 10% (T4), PDA 70% + OE 20% + TE 10% (T5) on mycelial growth diameter (MGD) and linear growth rate (MLGR) of Pleurotus ostreatus var. florida was compared to the one of PDA used as control (T1) at 22, 25 and 28°C inoculation temperatures. Optimum MGD was observed at 28°C in T1 (day 2), while it was significantly increased by 1.1–1.4 folds (days 4, 6, 8) in culture media containing plant extracts nevertheless the inoculation temperature. MLGR was improved by 102%–145% in olive/tea culture media compared to PDA nevertheless the inoculation temperature. Optimum growth induction index (GII) was observed in T3 (22.2%) at 22°C, T5 (21.9%) at 25°C and T4 (18.2%) at 28°C. These findings suggest the combination of olive and tea leaves extracts in the production of Pleurotus ostreatus .