A FRET-based fluorescent Zn<sup>2+</sup>sensor: 3D ratiometric imaging, flow cytometric tracking and cisplatin-induced Zn<sup>2+</sup>fluctuation monitoring
Hongxia Xu, Chengcheng Zhu, Yuncong Chen, Yang Bai, Zhong Han, Shankun Yao, Yang Jiao, Hao Yuan, Weijiang He, Zijian Guo
Abstract
A Zn<sup>2+</sup>sensor exhibiting 3D ratiometric imaging and flow cytometric ability was constructed based on the FRET mechanism, and cisplatin-induced endogenous labile Zn<sup>2+</sup>fluctuations were monitored in real time.
Topics & Concepts
Förster resonance energy transferFluorescenceZincChemistryTracking (education)EndogenyCisplatinRadiochemistryAnalytical Chemistry (journal)PhysicsOpticsBiochemistryChromatographyBiologyChemotherapyPsychologyGeneticsPedagogyOrganic chemistryAdvanced biosensing and bioanalysis techniquesMolecular Sensors and Ion DetectionNanocluster Synthesis and Applications