Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells
Jeong Seok Lee, Kijong Yi, Young Seok Ju, Eui‐Cheol Shin
Abstract
. However, the cryopreserved and thawed sample had a specific cluster with up-regulation of genes for heat shock proteins. Caution may be warranted in interpreting the character of any cluster of cells with heat shock-related properties when cryopreserved and thawed samples are used for scRNA-seq.
Topics & Concepts
CryopreservationPeripheral blood mononuclear cellTranscriptomeAndrologyFOXP3BiologyHeat shock proteinCell biologyComputational biologyImmunologyGeneGene expressionMedicineGeneticsEmbryoImmune systemIn vitroImmune Cell Function and InteractionT-cell and B-cell ImmunologyCAR-T cell therapy research