A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir
Claire Levy, Sean M. Hughes, Pavitra Roychoudhury, Daniel B. Reeves, Chelsea Amstuz, Haiying Zhu, Meei‐Li Huang, Yulun Wei, Marta E. Bull, Noah A.J. Cassidy, Jan McClure, Lisa M. Frenkel, Mars Stone, Sonia Bakkour, Elizabeth R. Wonderlich, Michael P. Busch, Steven G. Deeks, Joshua T. Schiffer, Robert W. Coombs, Dara A. Lehman, Keith R. Jerome, Florian Hladik
Abstract
T cells are faster for intact than for defective proviruses, and intact provirus frequencies are similar in mucosal and circulating T cells.
Topics & Concepts
Digital polymerase chain reactionHuman immunodeficiency virus (HIV)Measure (data warehouse)VirologyBiologyComputational biologyPolymerase chain reactionComputer scienceGeneBiochemistryDatabaseHIV Research and TreatmentBacteriophages and microbial interactionsCRISPR and Genetic Engineering