Physical confinement in alginate cryogels determines macrophage polarization to a M2 phenotype by regulating a STAT-related mRNA transcription pathway
Zhenzhen Liu, Nian-Yuan Xu, Meiling Wang, Ruizhi Tang, Xi‐Qiu Liu
Abstract
, resulting in the anti-inflammation effects. High-throughput RNA-seq analysis demonstrates that the mRNA surveillance pathway is key in the polarization process, and four primary transcription factors (PPAR-γ, STAT6, NF-κB, and STAT1) participate probably by competition in DNA binding to regulate M2-related gene expression. This study confirms that enough physical space inside is necessary to promote M2 polarization for the anti-inflammatory performance, which can be applied widely in the fields of tissue engineering and regenerative medicine.
Topics & Concepts
Macrophage polarizationCell biologyIn vivoChemistrySTAT6Transcription (linguistics)Polarization (electrochemistry)InflammationstatGene expressionBiomaterialPorositySTAT3BiophysicsPhenotypeTranscription factorPhosphorylationGeneNanotechnologyMaterials scienceBiologyImmunologyBiochemistryBiotechnologyPhysical chemistryPhilosophyLinguisticsOrganic chemistryImmune cells in cancerFibroblast Growth Factor ResearchNeonatal Respiratory Health Research