New Fluorescent Probes for the Sensitive Determination of Glyphosate in Food and Environmental Samples
Fang Sun, Liu Yang, Shijie Li, Yubo Wang, Ludi Wang, Ping Li, Fei Ye, Ying Fu
Abstract
In this paper, a dual-functional probe, 2-(benzothiazol)-4-(3-hydroxy-4-methylphenyl) imino phenol (BHMH), was synthesized and characterized for the simultaneous detection of Cu2+ and Fe3+ in dimethyl sulfoxide/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (DMSO/HEPES) (1:4, v/v, pH = 6.0). The limits of detections (LODs) for Cu2+ and Fe3+ were 9.05 and 48 nM, respectively. Based on the competitive coordination, the complex BHMH-Cu2+/Fe3+ exhibited good sensitivity and selectivity for glyphosate. The LODs of BHMH-Cu2+ and BHMH-Fe3+ for glyphosate were 0.41 and 0.63 μM, respectively. The probe quantitatively detected glyphosate in tap water, Songhua River water, local water and soil, and food samples. The colorimetric on-site glyphosate sensing through the probe BHMH-Cu2+ was also studied based on smartphones. BHMH and BHMH-Cu2+/Fe3+ exhibited outstanding imaging capabilities for Cu2+, Fe3+, and glyphosate in living cells with low cytotoxicity, especially the first time for glyphosate.