The Efflux Pump MexXY/OprM Contributes to the Tolerance and Acquired Resistance of Pseudomonas aeruginosa to Colistin
Hélène Puja, Arnaud Bolard, Aurélie Noguès, Patrick Plésiat, Katy Jeannot
Abstract
The intrinsic resistance of Pseudomonas aeruginosa to polymyxins in part relies on the addition of 4-amino-4-deoxy- l -arabinose (Ara4N) molecules to the lipid A of lipopolysaccharide (LPS), through induction of operon arnBCADTEF-ugd ( arn ) expression. As demonstrated previously, at least three two-component regulatory systems (PmrAB, ParRS, and CprRS) are able to upregulate this operon when bacteria are exposed to colistin. In the present study, gene deletion experiments with the bioluminescent strain PAO1:: lux showed that ParRS is a key element in the tolerance of P. aeruginosa to this last-resort antibiotic (i.
Topics & Concepts
Pseudomonas aeruginosaEffluxColistinMicrobiologyPseudomonadalesPseudomonadaceaeBiologyMCR-1ChemistryBacteriaAntibioticsEnterobacteriaceaeEscherichia coliGeneticsGeneAntibiotic Resistance in BacteriaAntibiotics Pharmacokinetics and EfficacyBacterial biofilms and quorum sensing