Solvent‐Selective Fluorescence Sensing of Mg<sup>2+</sup> and Al<sup>3+</sup> Ions by Pincer‐Type NNO Schiff Base Ligand: An Experimental and DFT Optimized Approach
Keshav Dev, Shiva Singh, Shakshi Bhardwaj, Prashant Kukreti, Dakuri Ramakanth, Pankaj Kumar, Saakshi Saini, Partha Roy, Vimal Chandra Srivastava, Kaushik Ghosh, Pradip K. Maji
Abstract
Abstract A newly developed dual‐functional fluorescence sensing probe (phenylhydrazinyl pyridine) Schiff base (SB) has been designed with good selectivity for distinguishing Mg 2+ and Al 3+ metal ions in different solvent solutions. SB exhibits quick and visual turn‐on fluorescence enhancement in response to Mg 2+ and Al 3+ detection. The addition of Mg 2+ in ACN–HEPES buffer (1 : 1, v/v, pH 7.2) at (λ max =390 nm) and Al 3+ in MeOH–HEPES buffer (1 : 1, v/v, pH 7.2) at (λ max =360 nm) resulted in significant enhancement of fluorescence, up to 7–9 times. These low detection limits of 7.1×10 −6 M (7.1 μM) and 5.15×10 −7 M (0.51 μM) for Mg 2+ and Al 3+ , respectively, have been achieved by this solvent‐controlled platform. Due to the sensing potential towards Mg 2+ , the probe was utilized as an imaging material for breast cancer cells. 1 H‐NMR studies were utilized to explore SB's sensing mechanism through turn‐on fluorescence. Density functional theory (DFT) calculations were utilized to validate optimized SB and its intricate geometries, which govern the sensing mechanism in the solvent environment. Such a probe has extensive potential applications in bioimaging and the assessment of the quality of wastewater.