Commanding the Commander: structure of a key protein machinery in endosomal trafficking
Xin Yong, Chunzhuang Zhou, Daniel D. Billadeau, Da Jia
Abstract
In a recent study published in Cell 1 and two bioRxiv pre-prints, 2 , 3 three studies investigate the structure of the endosomal Commander complex through a combination of X-ray crystallography, cryogenic-electron microscopy (Cryo-EM), Alphafold predictions and extensive site-directed mutagenesis (Fig. 1a ), which sheds lights on the molecular characteristics of this evolutionarily-conserved protein machinery, and enables the mapping of mutations causing X-linked intellectual disability (XILD) and Ritscher-Schinzel syndrome (RSS). Fig. 1 a The structure model of the Commander complex (Model Archive: ma-ri7tb), adapted from graphic abstract of the Cell study. 1 b The structural models of the SNX3-Retromer (PDB:7BLP) and SNX-BAR-Retromer complex (PDB:6H7W) on the membrane. c Comparison of Retriever and Retromer Full size image