The role of the biotin linker in polymer antibody mimetics, iBodies, in biochemical assays
Vladimír Šubr, Tereza Ormsby, Pavel Šácha, Jan Konvalinka, Tomáš Etrych, Libor Kostka
Abstract
We investigated the effect of a linker between the polymer backbone and biotin, and the influence of the number of biotins per polymer chain as well as the biotin position on the polymer chain on the efficacy of the ELISA or pull-down assays.
Topics & Concepts
BiotinLinkerPolymerChemistryCombinatorial chemistryBiochemistryPolymer chemistryBiophysicsOrganic chemistryBiologyComputer scienceOperating systemMonoclonal and Polyclonal Antibodies ResearchBiotin and Related StudiesAdvanced Biosensing Techniques and Applications