Facile Fabrication of Fe <sub>3</sub> O <sub>4</sub> @poly(acrylic) Acid Based Ferrofluid with Magnetic Resonance Imaging Contrast Effect
The Tam Le, Hoa Du Nguyen, Thi Ngoc Linh Nguyen, Thi Hong Tuyet Phan, Quang Ho Dinh, Thien Vuong Nguyen, Thi Thu Hiep Le, Thi Kim Oanh Vuong, Khoa Hai Le, Duong Duc La, Trong Lu Le, Тран Дай Лам
Abstract
Abstract Magnetic resonance imaging (MRI) has emerged as one of the most promising techniques, which employs nanoscience and imaging technology, for early diagnosis of the cancers. In this work, magnetic nanoparticles of Fe 3 O 4 @ poly(acrylic) acid (PAA) were synthesized using a low cost thermal decomposition method from iron ions precursor and utilized as a signal agent for MRI imaging. The Fe 3 O 4 magnetic nanoparticles obtained from FeCl 2 .4H 2 O precursor at the reaction temperature of 315 °C in 1‐octadcene solvent are of spherical shape with average diameter of 8.4 nm and high saturation magnetization (M s ) of 60.05 emu/g. After phase transferred with PAA, the magnetic Fe 3 O 4 @PAA fluid is highly stable in a wide range of pHs solution (5 ‐ 9) and wide range of NaCl concentrations (50 ‐ 300 mM) with zeta potential of −35.7 mV. The prepared Fe 3 O 4 @PAA fluid showed noncytotoxicity to the Hep‐G2, MCF‐7, RD cancer cell line and Vero healthy cell line. MRI in‐vitro results showed that transverse relaxation rate r 2 was around 158.4 mM −1 s −1 and the signal intensity was not affected by the pHs solution and NaCl concentrations. In‐vivo test on rabbit revealed the clear observation of rabbit's body parts after injection of the Fe 3 O 4 @PAA magnetic fluid with retention time of 90 minutes in the body.