<i>Nigella sativa</i> oil protects against emamectin benzoate‐Induced neurotoxicity in rats
Doaa A. Madkour, Mohamed M. Ahmed, Sahar H. Orabi, Samy Sayed, Reda M. S. Korany, Hanem K. Khalifa
Abstract
Abstract This study evaluated the ameliorative impact of Nigella sativa oil (NSO) on emamectin benzoate (EMB) neurotoxicity. Thirty‐five male rats were randomly allocated into 5 groups (n = 7). G1 (control): received distilled water; G2: received NSO (3 ml. Kg −1 B.W.) for 6 weeks; G3: received EMB (9 mg kg −1 B.W.) for 6 weeks; G4: was co‐treated with NSO and EMB for 6 weeks; G5: was treated with EMB for 4 weeks then, received NSO for 2 weeks. All treatments were given orally every other day. EMB increased serum urea, creatinine levels; brain dopamine, serotonin, malondialdehyde levels; brain expression levels of caspase 3 and TNF‐α. While, it decreased serum total protein, albumin, brain GABA, AChE, GSH‐Px, CAT, and SOD levels. Histopathological findings revealed hemorrhage, congestion, severe degeneration, and edema of the brain tissues. NSO reversed the EMB‐induced biochemical and histopathological alterations. This NSO effect is mostly due to its antioxidant, antiinflammatory, and antiapoptotic activities. These findings suggest NSO as a potential protective and therapeutic agent for EMB‐induced neurotoxicity.