Serial flow cytometry in an inertial focusing optofluidic microchip for direct assessment of measurement variations
Matthew DiSalvo, Paul N. Patrone, Anthony J. Kearsley, Gregory A. Cooksey
Abstract
, and analysis yields better than 99.9%. These achievements were enabled by a novel hybrid inertial and hydrodynamic particle focuser to tightly control particle positions and velocities. The cytometer identified ideal flow conditions with fluorescence area measurement precision on the order of 1% and characterized tradeoffs between precision, throughput, and analysis yield. The serial cytometer is anticipated to improve single-cell measurements through estimation (and subsequent control) of uncertainty contributions from various other instrument parameters leading to overall improvements in the ability to better classify sample composition and to find rare events.
Topics & Concepts
RepeatabilityInstrumentation (computer programming)Accuracy and precisionFlow (mathematics)Particle (ecology)ThroughputVolumetric flow rateBiological systemInertial measurement unitComputer scienceNanotechnologyMaterials sciencePhysicsMechanicsChemistryMathematicsChromatographyArtificial intelligenceStatisticsGeologyOceanographyWirelessOperating systemTelecommunicationsBiologyMicrofluidic and Bio-sensing TechnologiesDigital Holography and MicroscopyCell Image Analysis Techniques