Pulse Dipolar EPR Reveals Double-Histidine Motif Cu <sup>II</sup> –NTA Spin-Labeling Robustness against Competitor Ions
Joshua L. Wort, Swati Arya, Katrin Ackermann, Alan J. Stewart, Bela E. Bode
Abstract
-NTA at >1000-fold molar excess, and high nM binding affinity is surprisingly retained under acidic and basic conditions even though room temperature affinity shows a stronger pH dependence. This indicates the strategy is well-suited for diverse biological applications, with the requirement of other metal ion cofactors or slightly acidic pH not necessarily being prohibitive.
Topics & Concepts
Nitrilotriacetic acidChemistryHistidineIsothermal titration calorimetryElectron paramagnetic resonanceCrystallographyChelationNuclear magnetic resonanceInorganic chemistryPhysical chemistryBiochemistryAmino acidPhysicsElectron Spin Resonance StudiesMetal complexes synthesis and propertiesLanthanide and Transition Metal Complexes