ARHGEF3 regulates skeletal muscle regeneration and strength through autophagy
Jae‐Sung You, Nilmani Singh, Adriana Reyes‐Ordoñez, Nidhi Khanna, Zehua Bao, Huimin Zhao, Jie Chen
Abstract
(Cell Reports 34, 108594-1–108594-11.e1–e4; January 5, 2021) In the originally published version of this article, two rows of antibody information (anti-pSer9-GSK3β and anti-GSK3β) were missing. They now appear here and in the Key Resources Table and the Western blotting section of the STAR Methods in the corrected article online. The authors regret this error.Tabled 1Rabbit monoclonal anti-pSer9-GSK3βCell Signaling TechnologyCat#5558; Clone D85E12; RRID: AB_10013750Mouse monoclonal anti-GSK3βCell Signaling TechnologyCat#9832; Clone 3D10; RRID: AB_10839406 Open table in a new tab (BA-D5), type IIA (SC-71), type IIX (6H1), type IIB (BF-F3), and sarcomere (MF 20) from Developmental Studies Hybridoma Bank; anti-COX IV (4844), Cytochrome c (4272), pSer473-Akt (9271), pThr308-Akt (9275), Akt (9272), pThr346-NDRG1 (3217), NDRG1 (9408), pSer9GSK3β (5558), GSK3β (9832), LC3A/B (4108), p62 (23214), RhoA (2117), and GAPDH (2118) from Cell Signaling Technology; anti-pSer657-PKCa (06-822) from MilliporeSigma; anti-PKCa (P4334) from Sigma-Aldrich; anti-F4/80 (MCA497G) from Bio-Rad; anti-c-myc (MMS-150R) from Covance; peroxidase-conjugated anti-rabbit (115-036-003), anti-mouse (111-036-003), anti-mouse light chain specific (115-035-174), and anti-rat (172-035-153) IgG antibodies from Jackson Immuno Research Laboratories. ARHGEF3 Regulates Skeletal Muscle Regeneration and Strength through AutophagyYou et al.Cell ReportsJanuary 05, 2021In BriefPost-injury regeneration and aging significantly affect skeletal muscle function. One of the mechanisms governing these processes is autophagy. You et al. identify ARHGEF3 as an endogenous inhibitor of autophagy in muscles after injury as well as during aging. Through autophagy, ARHGEF3 deficiency enhances muscle regeneration and prevents age-related muscle weakness. Full-Text PDF Open Access