Site-Specific <i>N</i>-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry
Laura Pont, Valeriia Kuzyk, Fernando Benavente, Victòria Sanz‐Nebot, Oleg A. Mayboroda, Manfred Wuhrer, Guinevere S. M. Lageveen‐Kammeijer
Abstract
-glycosylation sites between primary adenocarcinoma and metastatic tumor in regard to branching, bisection, sialylation, and fucosylation. Those features, if further investigated in a targeted manner, may pave the way toward improved diagnostics and monitoring of colorectal cancer progression and recurrence. Raw mass spectrometric data and Skyline processed data files that support the findings of this study are available in the MassIVE repository with the identifier MSV000086774 [DOI: 10.25345/C5Z50X].
Topics & Concepts
GlycosylationCarcinoembryonic antigenFucosylationChemistryTandem mass spectrometryBiochemistryGlycomicsGlycanGlycoproteinMass spectrometryChromatographyCancerBiologyGeneticsGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies ResearchGalectins and Cancer Biology