<p>Co-Existence of <em>mcr-1</em> and <em>bla</em><sub>NDM-5</sub> in an <em>Escherichia</em> <em>coli</em> Strain Isolated from the Pharmaceutical Industry, WWTP</p>
Huiming Han, Wenhong Liu, Xinjie Cui, Xu Cheng, Xiawei Jiang
Abstract
Abstract: The emergence of the plasmid-borne colistin-resistant gene ( mcr-1 ) poses a great threat to human health. What is worse, the recent observations of the co-existence of mcr-1 with other antimicrobial resistance genes in some bacteria cause further concern. Here, we present the first report of a wild Escherichia coli strain that co-carries an mcr-1 encoding phage-like IncY plasmid (pR15_MCR-1) and a bla NDM-5 encoding IncX3 plasmid (pR15_NDM-5) from a pharmaceutical industry, wastewater treatment plant, in China. This study highlights the spreading of E. coli carrying both mcr-1 and bla NDM-5 genes in the pharmaceutical industry. Importance: Escherichia coli strains that carry both mcr-1 and bla NDM-5 genes are of great health concern and are already found in humans and animals worldwide, yet there is a paucity of observations of this resistant strain in the environment. Here we present the first isolation of an E. coli strain (R15) that co-carries mcr-1 and bla NDM-5 genes from a wastewater treatment plant in China. Whole-genome sequencing indicated that R15 harbored two plasmids, pR15_MCR-1 and pR15_NDM-5, that carry mcr-1 and bla NDM-5 , respectively. The observation of this wild-derived E. coli strain that carries mcr-1 and bla NDM-5 genes simultaneously calls for the urgency to improve monitoring and reducing its further spreading. Keywords: mcr-1 , bla NDM-5 , wastewater treatment plant, Escherichia coli