A CRISPR-Cas12a integrated SERS nanoplatform with chimeric DNA/RNA hairpin guide for ultrasensitive nucleic acid detection
Bohan Yin, Qin Zhang, Xinyue Xia, Chuanqi Li, Willis Kwun Hei Ho, Jiaxiang Yan, Yingying Huang, Honglian Wu, Pui Wang, Changqing Yi, Jianhua Hao, Jianfang Wang, Honglin Chen, Siu Hong Dexter Wong, Mo Yang
Abstract
Our work not only offers a platform to sensitively and selectively probe nucleic acids without pre-amplification but also provides new insights into the design of the CRISPR-Cas12a/SERS integrated system to resolve the steric hindrance of nanomaterials for constructing biosensors.
Topics & Concepts
Nucleic acidCRISPRNanomaterialsNanotechnologyChemistryNucleic acid detectionDNANucleic acid quantitationDetection limitRNAMaterials scienceBiochemistryChromatographyGeneAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionCRISPR and Genetic Engineering