Rerouting of ribosomal proteins into splicing in plant organelles
Chuande Wang, Rachel Fourdin, Martine Quadrado, Céline Dargel-Graffin, Dimitri Tolleter, David Macherel, Hakim Mireau
Abstract
uL18-Like proteins are targeted to either mitochondria or chloroplasts. While two members of the family are found in organelle ribosomes, we show here that two uL18-type proteins function as factors necessary for the splicing of certain mitochondrial and plastid group II introns. These two proteins do not cosediment with mitochondrial or plastid ribosomes but instead associate with the introns whose splicing they promote. Our study thus reveals that the RNA-binding capacity of uL18 ribosomal proteins has been repurposed to create factors that facilitate the splicing of organellar introns.
Topics & Concepts
IntronRibosomeRNA splicingRibosomal proteinBiologyGeneticsRibosomal RNAOrganellePlastidGeneGroup I catalytic intron5S ribosomal RNAComputational biologyCell biologyRNAChloroplastRNA and protein synthesis mechanismsRNA modifications and cancerRNA Research and Splicing