Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method
Tom Lawrenson, Nicola Atkinson, Macarena Forner, Wendy Harwood
Topics & Concepts
Medicago truncatulaBiologyCas9Genome editingRhizobiaAgrobacteriumGene knockoutMutantGeneTransformation (genetics)GeneticsCRISPRTransgeneMedicagoComputational biologySymbiosisBacteriaCRISPR and Genetic EngineeringInsect symbiosis and bacterial influencesPlant tissue culture and regeneration