Two-photon fluorescent probes for detecting the viscosity of lipid droplets and its application in living cells
Huiying Chen, Jianzhi Zhao, Junzhi Lin, Baoli Dong, Hui Li, Bing Geng, Mei Yan
Abstract
the typical ICT system of D-π-A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluorescence intensity of CIV increased by 13-fold, showing sensitivity and specificity towards viscosity. In addition, CIV showed low toxicity and excellent biocompatibility in cytotoxicity tests, and was successfully used for living cell LD imaging. Taken together, the results widen the way for the development of novel fluorescent probe-based the visualization LDs and detection in solutions, physiology and pathology.
Topics & Concepts
FluorescenceViscosityLipid dropletChemistryNanotechnologyBiophysicsMaterials sciencePhysicsOpticsBiologyBiochemistryComposite materialLipid metabolism and biosynthesisMetabolomics and Mass Spectrometry StudiesLipid Membrane Structure and Behavior