Channelling carbon flux through the <i>meta</i> ‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in <i>Pseudomonas putida</i> H
José Manuel Borrero‐de Acuña, Izabook Gutiérrez‐Urrutia, Cristian Hidalgo-Dumont, Carla Aravena-Carrasco, Matias Orellana‐Saez, Nestor Palominos-Gonzalez, Jozef B. J. H. van Duuren, Viktoria Wagner, Lars Gläser, Judith Becker, Michael Kohlstedt, Flavia C. Zacconi, Christoph Wittmann, Ignacio Poblete‐Castro
Abstract
PHA. The balancing of carbon flux between the parallel catechol-degrading routes emerges as an important strategy to prevent intermediate accumulation and elevate mcl-PHA production in P. putida H and, as shown here, sets the next level to derive this sustainable biopolymer from lignin hydrolysates and aromatics.
Topics & Concepts
LigninPseudomonas putidaChannellingChemistryFlux (metallurgy)Cleavage (geology)Carbon fluxCarbon fibersOrganic chemistryComputer scienceMaterials scienceBiologyEnzymeAlgorithmFracture (geology)Composite materialEcologyComposite numberIonEcosystembiodegradable polymer synthesis and propertiesBiofuel production and bioconversionMicrobial Metabolic Engineering and Bioproduction