pHLARE: a new biosensor reveals decreased lysosome pH in cancer cells
Bradley A. Webb, Francesca M. Aloisio, Rabab A. Charafeddine, Jessica Cook, Torsten Wittmann, Diane L. Barber
Abstract
porter), which localizes predominantly in lysosomes, has a dynamic range of pH 4.0 to 6.5, and can be stably expressed in cells. Using pHLARE we show decreased pHlys with inhibiting activity of the mammalian target of rapamycin complex 1 (mTORC1). Also, cancer cells from different tissue origins have a lower pHlys than untransformed cells, and stably expressing oncogenic RasV12 in untransformed cells is sufficient to decrease pHlys. pHLARE is a new tool to accurately measure pHlys for improved understanding of lysosome dynamics, which is increasingly considered a therapeutic target.
Topics & Concepts
BiologyLysosomeBiosensorCancer cellCell biologyBiochemistryCancerEnzymeGeneticsATP Synthase and ATPases ResearchCalcium signaling and nucleotide metabolismGlycosylation and Glycoproteins Research