A structure-guided engineering strategy targeting the F/G region enhances tryptamine 5-hydroxylase activity for efficient serotonin production
Ling Gao, Peng Wei, Wenzhao Xu, Xiaole Xia
Topics & Concepts
TryptamineChemistryProtein engineeringSteric effectsBiochemistryEnzymeTryptophan hydroxylaseMetabolic engineeringHeterologous expressionTryptophanSubstrate (aquarium)BiosynthesisStereochemistryCytochrome P450HydroxylationActive siteDirected evolutionSynthetic biologyHeterologousCofactorBiophysicsSaturated mutagenesisCombinatorial chemistryPharmacogenetics and Drug MetabolismChemical synthesis and alkaloidsMicrobial Natural Products and Biosynthesis