Orthogonal protocols for DNA extraction from filamentous fungi
Benjamin H. Conlon, Suzanne Schmidt, Michael Poulsen, Jonathan Z. Shik
Abstract
There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).
Topics & Concepts
DNA extractionBiologyDNAExtraction (chemistry)genomic DNAComputational biologyAscomycotaGeneticsPolymerase chain reactionGeneChemistryChromatographyPlant Pathogens and Fungal DiseasesMycorrhizal Fungi and Plant InteractionsYeasts and Rust Fungi Studies