Immunohistochemistry for ESR2 with a Mouse Monoclonal Antibody (PPZ0506)
Masahiro Morishita, Shimpei Higo, Yujiro Hattori, Mina Ozawa, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii
Abstract
Lack of reliable antibodies against estrogen receptor (ESR2), a nuclear estrogen receptor, has stalled ESR2 research.Thus, the recent discovery of a well-validated antibody against human ESR2 (PPZ0506) 1 provided momenta to develop new ESR2 detection systems.To utilize PPZ0506 for immunohistochemical detection of rodent ESR2 proteins, our previous studies confirmed its specific cross-reactivity against rat and mouse ESR2 proteins and optimized protocols for immunohistochemical staining of rat and mouse sections with PPZ0506 2-4 .Here, we present the immunohistochemical procedures optimized in our previous study with some modifications (Supplementary Text; https://doi.org/10.1272/jnms.JNMS.2023_90-209)and show representative photomicrographs obtained following the procedures in Figure 1, 2. Specific immunoreactive signals were observed in granulosa cells of rat and mouse ovaries (Fig. 1A), epithelial cells of rat dorsal and ventral prostate (Fig. 1B), and neurons of rat and mouse brain subregions containing the principal nucleus of the bed nucleus of the stria terminalis, paraventricular nucleus, and posterodorsal part of the medial amygdala (Fig. 2).