Increases in mRNA and Protein Levels of the Genes for the Actin-Binding Proteins Profilin, Fascin, and Ezrin Promote Metastasis in Non-Small Cell Lung Cancer
Е. С. Колегова, Г. В. Какурина, Dmitriy N Kostromitskiy, А. Yu. Dobrodeev, И. В. Кондакова
Abstract
The actin-binding proteins profilin, fascin, and ezrin were tested for involvement in metastasis of non-small cell lung cancer (NSCLC). The levels of the PFN1, FSCN1, and EZR mRNAs and respective proteins were determined by real-time PCR and Western blotting; tumor and adjacent normal lung tissue samples were obtained from 46 NSCLC patients. Patients with lymphatic metastasis had higher expression levels of the profilin, fascin, and ezrin mRNAs and the profilin and fascin proteins. Both mRNA and protein expression levels increased in patients with distant metastasis. The molecules may serve as predictors to evaluate the prognosis in NSCLC.
Topics & Concepts
EzrinFascinMessenger RNAMetastasisProfilinActinCancer researchLung cancerBlotBiologyGeneActin cytoskeletonCellMolecular biologyCancerCell biologyMedicinePathologyCytoskeletonGeneticsCellular Mechanics and InteractionsNeurofibromatosis and Schwannoma CasesMicrotubule and mitosis dynamics