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Investigating the effectiveness of a bacterial self-healing mechanism for repairing cracks in sustainable cement mortar at low temperatures

Mahmoud A. Elshazly, Ahmed A. Elakhras, Ahmed A. Elshami, Seleem Ahmad, Mohamed A.R. Elmahdy

2025Results in Engineering14 citationsDOIOpen Access PDF

Abstract

• Bacterial self-healing for cracks in sustainable cement mortar • Performance of bacteria types at different lower temperatures. • Healing ratio at room and lower temperatures. • Behavior of Bacillus Megaterium and Bacillus Sphaericus bacteria at sub-zero temperatures. Mortar forms a fundamental constituent of concrete, resulting in similar behavior and drawbacks between them. In this study, we investigate one of these drawbacks (cracks). This study investigates bacterial self-healing efficiency at different temperatures RT (24°C), Zero, and Sub-Zero (-16 ± 2 °C, and – RT, (- 24 °C)), bacteria type, concentration, and percent on self-healing of cracks in cement mortar. The two distinct strains of bacteria were Bacillus Megaterium (BM) and Bacillus Sphaericus (BS), with varying concentrations of 0 %, 1%, and 2.5%. The efficiency of the bacterial strains was evaluated using a variety of techniques, such as compressive and flexural strength tests, energy-dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and X-ray diffraction (XRD). It was observed that RT and Zero temperature at 2.5 % of BS or BM had the same behavior for crack-healing efficiency by 100% with an improvement in the mechanical strength of the mortar. However, BM survived and kept healing the cracks in Sub-Zero temperatures, with a healing efficiency of 38.37% after 56 days at the temperature of -16 ± 2 °C. Calcium carbonate was also detected by the SEM and EDS tests, indicating that the bacteria were actively taking part in the healing process for themselves.

Topics & Concepts

Self-healingMortarMechanism (biology)CementMaterials scienceComposite materialMedicinePathologyPhilosophyEpistemologyAlternative medicineMicrobial Applications in Construction MaterialsConcrete and Cement Materials ResearchBuilding materials and conservation