Required Gene Set for Autotrophic Growth of <i>Clostridium autoethanogenum</i>
Craig Woods, Christopher M. Humphreys, Claudio Tomi-Andrino, Anne M. Henstra, Michael Köpke, Séan D. Simpson, Klaus Winzer, Nigel P. Minton
Abstract
into chemicals and fuels at an industrial scale. This allowed the determination of gene essentiality under heterotrophic and autotrophic growth, providing insights into the utilization of CO as a sole carbon and energy source. The strategies implemented are translatable and will allow others to apply transposon insertion sequencing to other microbes where DNA transfer has until now represented a barrier to progress.
Topics & Concepts
Transposable elementBiologyPlasmidGeneticsGenomeTransposaseGeneComputational biologyBacterial genome sizeTransformation (genetics)Microbial Metabolic Engineering and BioproductionMicrobial Fuel Cells and BioremediationBiofuel production and bioconversion