Fungal Integrated Histomolecular Diagnosis Using Targeted Next-Generation Sequencing on Formalin-Fixed Paraffin-Embedded Tissues
Alexis Trécourt, Méja Rabodonirina, Claire Mauduit, Alexandra Traverse‐Glehen, Mojgan Devouassoux‐Shisheboran, David Meyronet, Frédérique Dijoud, Christophe Ginévra, Emmanuelle Chapey, Emilie Josse, Patrícia Martins-Simões, Abderrazzak Bentaher, Damien Dupont, Charline Miossec, Florence Persat, Martine Wallon, Tristan Ferry, Félix Pham, Bruno Simon, Jean Ménotti
Abstract
Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide a genus and/or species identification. The objective of the present study was to develop targeted next-generation sequencing (NGS) on formalin-fixed tissue samples (FTs) to achieve a fungal integrated histomolecular diagnosis.
Topics & Concepts
MucoralesSanger sequencingBiologyDNA extractionAspergillus fumigatusHigh Resolution MeltAspergillusPolymerase chain reactionPrimer (cosmetics)AspergillosisDNA sequencingMicrobiologyMucormycosisMedicineGeneticsPathologyDNAChemistryGeneImmunologyOrganic chemistryAntifungal resistance and susceptibilityPlant Pathogens and Fungal DiseasesFungal Infections and Studies